| Chymotrypsin Basic information |
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| Chymotrypsin Chemical Properties |
| Melting point |
127°C |
| storage temp. |
2-8°C |
| solubility |
Reconstitute in 1mM HCl. Soluble at 10mg/ml in 1mM HCl. 2mM calcium chloride serves as a stabilizer. Store aliquoted solutions at -20°C for up to a week. |
| form |
salt-free, lyophilized powder |
| color |
white |
| Merck |
13,2282 |
| EPA Substance Registry System |
Chymotrypsin (9004-07-3) |
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| Chymotrypsin Usage And Synthesis |
| Chemical Properties |
Lyophilized powder, dialyzed |
| Uses |
α-Chymotrypsin from bovine has been used in a study to inform proteasome inhibition in order to advance anticancer research. α-Chymotrypsin from bovine has also been used in a study that functionalized surface anchored poly(methylhydrosiloxane) thin films on oxidized silicon wafers. |
| General Description |
Chymotrypsin (Chymar) is extractedfrom mammalian pancreas and is used in cataractsurgery. A dilute solution is used to irrigate the posteriorchamber of the eye to dissolve the fine filaments that holdthe lens. |
| Purification Methods |
α-Chymotrypsin is crystallised twice from four-tenths saturated ammonium sulfate solution, then dissolved in 1mM HCl and dialysed against 1mM HCl at 2-4o. The solution is stored at 2o [Lang et al. J Am Chem Soc 80 4923 1958]. |
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| Chymotrypsin Preparation Products And Raw materials |
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